Hello my friends, today i will talk about the micro o nano world, is a very difficult topic for me, because i don't have many knowledge about this topic.
I try of explain the technique "UPLC"( ultra high performance liquid chromatography), well "ULPC" is a technique that comes of "HPLC"(high performance liquid chromatography) , both are chromatography techniques, the chromatography is a technique of compound separating and this is carried about product of the interaction of the different molecules with the two phases that make up the chromatography column (stationary phase and mobile phase), this technique have many variants depending on the type of stationary phase of molecule to study, but in all cases the process is simple we have a column and by it moves a liquid to carry or sample, which separated from the burials.
the different between "HPLC" and "UPLC", is the size of the particles that compose the stationary phase, going from 9-7 µm ( in "HPLC") to 1,5-0,8 µm ( in "ULPC"), this littles different in the size of the particles produce a big change in process efficiency and in the time of the chromatography, and this changes are good for the investigation work.in my career the "ULPC" ( and the "HPLC") are very used in process of search of compounds in low concentrations, for example toxic compounds, also we can used it to know the concentration of this compound, ( for example a drug).
this new system of chromatography have many potential for the future, being able to improve the processes, for example time work or efficiency of the processes
.
well, today i talk about this topic because i understand a bit, because saw it in classes of the analytic chemistry in my university and wanted to talk about something i knew.
I try of explain the technique "UPLC"( ultra high performance liquid chromatography), well "ULPC" is a technique that comes of "HPLC"(high performance liquid chromatography) , both are chromatography techniques, the chromatography is a technique of compound separating and this is carried about product of the interaction of the different molecules with the two phases that make up the chromatography column (stationary phase and mobile phase), this technique have many variants depending on the type of stationary phase of molecule to study, but in all cases the process is simple we have a column and by it moves a liquid to carry or sample, which separated from the burials.
the different between "HPLC" and "UPLC", is the size of the particles that compose the stationary phase, going from 9-7 µm ( in "HPLC") to 1,5-0,8 µm ( in "ULPC"), this littles different in the size of the particles produce a big change in process efficiency and in the time of the chromatography, and this changes are good for the investigation work.in my career the "ULPC" ( and the "HPLC") are very used in process of search of compounds in low concentrations, for example toxic compounds, also we can used it to know the concentration of this compound, ( for example a drug).
this new system of chromatography have many potential for the future, being able to improve the processes, for example time work or efficiency of the processes
.
well, today i talk about this topic because i understand a bit, because saw it in classes of the analytic chemistry in my university and wanted to talk about something i knew.
Wow chromatography is a very used technique and very important. The UPLC technique sounds like is the best.
ReplyDeleteAt school I had to talk about this technique
ReplyDelete